O INCREMENTO NO DIAGNÓSTICO DE INFECÇÕES DO TRATO URINÁRIO PELA INCLUSÃO DA COLORAÇÃO DE GRAM NA URINÁLISE

Autores

DOI:

https://doi.org/10.63845/cws37343

Palavras-chave:

exame de urina; cultura de urina; infecção do trato urinário; coloração de Gram; sedimento urinário

Resumo

O exame de urina é teste auxiliar para o diagnóstico de infecções do trato urinário (ITU). Esse apresenta baixa sensibilidade e especificidade na detecção de ITU e bacteriúria assintomática (BA). O estudo busca reforçar a relevância dos resultados da análise de urina com coloração de Gram nas lâminas de sedimentos de urina. Foram estudadas 235 amostras de urina coletadas em ambulatório da Faculdade de Medicina do ABC. Os resultados indicam maior positividade de ITU em exames de urina, coloração de Gram e urocultura (72,7%) no sexo masculino. Entre as amostras negativas para a cultura de urina, 11 foram positivas usando a coloração de Gram, sugerindo que esse método pode detectar ITU por bactérias exigentes ou BA. A concordância entre a coloração de Gram na urina e a cultura de urina foi de 94,98% e substancial (Kappa = 0,777), em oposição a 81,59% entre as análises de urina e cultura de urina e moderada (Kappa = 0,448). A análise de urina mostrou sensibilidade de 89,66% e especificidade de 80,48%, mas a coloração de Gram na urina mostrou 86,21% de sensibilidade e especificidade de 96,94%. A inclusão da coloração de Gram no exame de urina melhora a especificidade, aumenta a detecção de bactérias exigentes e casos de BA, levando a uma diminuição no número de falsos positivos e auxiliar na definição da antibioticoterapia sem a cultura de urina. Os resultados sugerem que a inclusão da coloração de Gram no exame de urina melhora o diagnóstico de ITU, sendo relevante em  procedimentos hospitalares.

Referências

Hirakauva E, Bianchi-Ferraro A, Zucchi E, Kajikawa M, Girão M, Sartori M, et al. Incidence of Bacteriuria after Urodynamic Study with or without Antibiotic Prophylaxis in Women with Urinary Incontinence. Rev Bras Ginecol e Obs / RBGO Gynecol Obstet. 2017;39(10):534–40. DOI: https://doi.org/10.1055/s-0037-1604066

Açik MN, Yurdakul NE, Çakici L, Onat N, Dogan Ö, Çetinkaya B. traT and CNF2 genes of Escherichia coli isolated from milk of healthy cows and sheep. Res Vet Sci. 2014;77(1):17–21. DOI: https://doi.org/10.1016/j.rvsc.2004.01.003

Roriz-Filho JS, Vilar, FC, Mota LM, et al. Infecção do trato urinário. Medicina Ribeirao

Preto Online. 2010; 43(2):118-25. DOI: https://doi.org/10.11606/issn.2176-7262.v43i2p118-125

Skjøt-Rasmussen L, Olsen SS, Jakobsen L, Ejrnæs K, Scheutz F, Lundgren B, et al. Escherichia coli clonal group A causing bacteraemia of urinary tract origin. Clin Microbiol Infect. 2013;19(7):656–61. DOI: https://doi.org/10.1111/j.1469-0691.2012.03961.x

Köves B, Cai T, Veeratterapillay R, Pickard R, Seisen T, Lam TB, et al. Benefits and Harms of Treatment of Asymptomatic Bacteriuria: A Systematic Review and Meta-analysis by the European Association of Urology Urological Infection Guidelines Panel. Eur Urol. 2017;72(6):865–8. DOI: https://doi.org/10.1016/j.eururo.2017.07.014

Eriksson I, Olofsson B, Gustafson Y, Fagerström L. Older women’s experiences of suffering from urinary tract infections. J Clin Nurs. 2014;23(9–10):1385–94. DOI: https://doi.org/10.1111/jocn.12422

Keren R, Shaikh N, Pohl H, Gravens-Mueller L, Ivanova A, Zaoutis L, et al. Risk factors for recurrent urinary tract infection and renal scarring. Pediatrics. 2015;136(1):e13–21. DOI: https://doi.org/10.1542/peds.2015-0409

Glaser AP, Schaeffer AJ. Urinary Tract Infection and Bacteriuria in Pregnancy. Urol Clin North Am [Internet]. 2015;42(4):547–60. Available from: http://dx.doi.org/10.1016/j.ucl.2015.05.004 DOI: https://doi.org/10.1016/j.ucl.2015.05.004

Dune TJ, Price TK, Hilt EE, Thomas-White KJ, Kliethermes S, Brincat C, et al. Urinary symptoms and their associations with urinary tract infections in urogynecologic patients. Obstet Gynecol. 2017;130(4):718–25. DOI: https://doi.org/10.1097/AOG.0000000000002239

Kistler CE, Zimmerman S, Scales K, Ward K, Weber D, Reed D, et al. The Antibiotic Prescribing Pathway for Presumed Urinary Tract Infections in Nursing Home Residents. J Am Geriatr Soc. 2017;65(8):1719–25. DOI: https://doi.org/10.1111/jgs.14857

Oyaert M, Delanghe J. Progress in automated urinalysis. Ann Lab Med. 2018;39(1):15–22. DOI: https://doi.org/10.3343/alm.2019.39.1.15

Nascimento DZ, Pickler MD, Marques GM, Trevisol FS, Martins ALO. Sediment examination of urine without physical-chemical alterations. J Bras Patol e Med Lab. 2018;54(3):177–82. DOI: https://doi.org/10.5935/1676-2444.20180031

De Toro-Peinado I, Concepción Mediavilla-Gradolph M, Tormo-Palop N, Palop-Borrás B. Microbiological diagnosis of urinary tract infections. Enferm Infecc Microbiol Clin [Internet]. 2015;33(S2):34–9. Available from: http://dx.doi.org/10.1016/S0213-005X(15)30013-6 DOI: https://doi.org/10.1016/S0213-005X(15)30013-6

Foxman B. Urinary tract infection syndromes. Occurrence, recurrence, bacteriology, risk factors, and disease burden. Infect Dis Clin North Am [Internet]. 2014;28(1):1–13. Available from: http://dx.doi.org/10.1016/j.idc.2013.09.003 DOI: https://doi.org/10.1016/j.idc.2013.09.003

Cai T, Koves B, Johansen TEB. Asymptomatic bacteriuria, to screen or not to screen - And when to treat? Curr Opin Urol. 2017;27(2):107–11. DOI: https://doi.org/10.1097/MOU.0000000000000368

Flokas ME, Andreatos N, Alevizakos M, Kalbasi A, Onur P, Mylonakis E. Inappropriate Management of Asymptomatic Patients with Positive Urine Cultures: A Systematic Review and Meta-analysis. Open Forum Infect Dis. 2017;4(4):1–10. DOI: https://doi.org/10.1093/ofid/ofx207

Ipe DS, Sundac L, Benjamin WH, Moore KH, Ulett GC. Asymptomatic bacteriuria: Prevalence rates of causal microorganisms, etiology of infection in different patient populations, and recent advances in molecular detection. FEMS Microbiol Lett. 2013;346(1):1–10. DOI: https://doi.org/10.1111/1574-6968.12204

Coulthard MG. Using urine nitrite sticks to test for urinary tract infection in children aged < 2 years: a meta-analysis. Pediatr Nephrol. 2019;(August 2018):1283–8. DOI: https://doi.org/10.1007/s00467-019-04226-6

Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H, Kobayashi I, et al. Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet. 2001;357(9264):1225–40. DOI: https://doi.org/10.1016/S0140-6736(00)04403-2

de Boer FJ, Gieteling E, van Egmond-Kreileman H, Moshaver B, van der Leur SJCM, Stegeman CA, et al. Accurate and fast urinalysis in febrile patients by flow cytometry. Infect Dis (Auckl) [Internet]. 2017;49(5):380–7. Available from: http://dx.doi.org/10.1080/23744235.2016.1274048 DOI: https://doi.org/10.1080/23744235.2016.1274048

Chotiprasitsakul D, Kijnithikul A, Uamkhayan A, Santanirand P. Predictive Value of Urinalysis and Recent Antibiotic Exposure to Distinguish Between Bacteriuria, Candiduria, and No-Growth Urine. Infect Drug Resist. 2021;14:5699–709. DOI: https://doi.org/10.2147/IDR.S343021

Kocer D, Sariguzel FM, Karakukcu C. Cutoff values for bacteria and leukocytes for urine sediment analyzer FUS200 in culture-positive urinary-tract infections. Scand J Clin Lab Invest. 2014;74(5):414–7. DOI: https://doi.org/10.3109/00365513.2014.900189

O’Neil E, Horney B, Burton S, Lewis PJ, Mackenzie A, Stryhn H. Comparison of wet-mount, Wright-Giemsa and Gram-stained urine sediment for predicting bacteriuria in dogs and cats. Can Vet J. 2013;54(11):1061–6.

Amaral L, Martins A, Spengler G, Molnar J. Efflux pumps of Gram-negative bacteria : what they do , how they do it , with what and how to deal with them. 2014;4(January):1–11. DOI: https://doi.org/10.3389/fphar.2013.00168

Way LI, Sullivan LA, Johnson V, Morley PS. Comparison of routine urinalysis and urine Gram stain for detection of bacteriuria in dogs. J Vet Emerg Crit Care. 2013;23(1):23–8. DOI: https://doi.org/10.1111/vec.12012

Yodoshi T, Matsushima M, Taniguchi T, Kinjo S. Utility of point-of-care Gram stain by physicians for urinary tract infection in children ≤36 months. Medicine (Baltimore). 2019;98(14):e15101. DOI: https://doi.org/10.1097/MD.0000000000015101

Kayalp D, Dogan K, Ceylan G, Senes M, Yucel D. Can routine automated urinalysis reduce culture requests? Clin Biochem [Internet]. 2013;46(13–14):1285–9. Available from: http://dx.doi.org/10.1016/j.clinbiochem.2013.06.015 DOI: https://doi.org/10.1016/j.clinbiochem.2013.06.015

Stata. Stata base reference manual - release 12. 2011. 2357 p.

Yang G, Sau C, Lai W, Cichon J, Li W. 蚊子网状进化HHS Public Access. 2015;344(6188):1173–8. DOI: https://doi.org/10.1126/science.1249098

Sanchez G V., Babiker A, Master RN, Luu T, Mathur A, Bordon J. Antibiotic resistance among urinary isolates from female outpatients in the United States in 2003 and 2012. Antimicrob Agents Chemother. 2016;60(5):2680–3. DOI: https://doi.org/10.1128/AAC.02897-15

Gupta K, Grigoryan L, Trautner B. In the clinic® urinary tract infection. Ann Intern Med. 2017;167(7):ITC49–64. DOI: https://doi.org/10.7326/AITC201710030

Knottnerus BJ, Geerlings SE, Van Charante EPM, Ter Riet G. Women with symptoms of uncomplicated urinary tract infection are often willing to delay antibiotic treatment: A prospective cohort study. BMC Fam Pract [Internet]. 2013;14(1):1. Available from: BMC Family Practice DOI: https://doi.org/10.1186/1471-2296-14-71

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02/04/2025

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O INCREMENTO NO DIAGNÓSTICO DE INFECÇÕES DO TRATO URINÁRIO PELA INCLUSÃO DA COLORAÇÃO DE GRAM NA URINÁLISE. (2025). Arquivos Catarinenses De Medicina, 53(3), 03-16. https://doi.org/10.63845/cws37343